Performance Data

Precision
Intra-assay	Mean [μmol/L]	SD [μmol/L]	CV [%]
Sample 1	5.41	0.129	2.38
Sample 2	10.2	0.085	0.83
Sample 3	199	1.19	0.60
Inter-assay	Mean [μmol/L]	SD [μmol/L]	CV [%]
Sample 1	5.39	0.076	1.42
Sample 2	10.4	0.159	1.54
Sample 3	201	1.64	0.82

Recovery of clinically significant bile acids
Recovery in aqueous bile acids [50 µM]	DiaSys Cycling Method		Competitor A Cycling Method		Competitor B Cycling Method
Recovery in aqueous bile acids [50 µM]	[µmol/L]	[%]	[µmol/L]	[%]	[µmol/L]	[%]
Glycocholic acid	53.4	107	36.2	72	38.9	78
Glycochenodeoxycholic acid	48.9	98	49.4	99	67.0	134
Taurochenodeoxycholic acid	51.8	104	48.5	97	57.2	114
Taurocholic acid	50.3	101	34.0	68	34.6	69
Chenodeoxycholic acid	53.5	107	51.9	104	75.8	152
Cholic acid	51.6	103	35.6	71	38.4	77
Deoxycholic acid	50.1	100	61.8	124	74.6	149
Taurodeoxycholic acid	47.3	95	56.6	113	56.8	114
Lithocholic acid	48.4	97	-	-	-	-

Total bile acids 21 FS